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. 2020 Sep 1;14(11):2890–2900. doi: 10.1038/s41396-020-00760-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–c Three images taken from a Z-slice of a colony of B12(pGFP) during predation by IR1 (unstained, lines of advance shown with white arrows). From left to right the three slices show B12 cells at the agar surface, then 5, and 10 µm heights. d Overview image assembled from multiple contiguous images showing IR1 penetrating a colony of B12(pGFP). IR1 (not stained, visible as dark root-like regions but with an overall invasion route of top right to bottom left) is moving into a colony of GFP-expressing B12. White arrows show the direction of movement of some of the IR1 masses. Propidium iodide (red) is staining damaged cells (predominantly B12) within 20 μm of the major lines of advance of IR1. The scale bar in (d) indicates 50 µm when applied to (a–c) and 80 µm when applied to (d).