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. 2020 Sep 1;14(11):2890–2900. doi: 10.1038/s41396-020-00760-6

Fig. 5. Competition by SC-deficient IR1 mutants.

Fig. 5

B12(pGFP) was mixed with IR1 (WT or transposon mutants) in the co-inoculation competition assay on ASWBLow agar. After 30 h the growth of B12 was assessed by fluorescence microscopy, capturing a series of 4 × 2.5 mm areas in triplicate (large images). Quantification of GFP was used to assess the degree to which IR1 inhibited the growth of B12 (Fig. S7). SC (structural colour) insets: two colony images for the same strain showing the appropriate strain cultured for 30 h on ASWBC agar (upper inset) or ASWBLow agar (lower inset). a WT IR1 strain. b M1 (gmp1::HiMar). c M5 (spoT5::HiMar)*. d M9 (acr9::HiMar). e M10 (mal10::HiMar). f M12 (sprF12::HiMar)*. g M16 (hypA16::HiMar)*. h M17 (gldiA17::HiMar)*. i M49 (mtr49::HiMar). j M51 (malT51::HiMar)*. k M52 (hypB52::HiMar)*. l M65 (hk65::HiMar). m M76 (ugd76::HiMar). n M160 (hypC160::HiMar). For strains marked * independent transposon insertions in the same gene were available and tested with similar results.