Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 20;30(11):1024–1042. doi: 10.1038/s41422-020-0374-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Center for Excellence in Molecular Cell Science, CAS 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a t-SNE plot of 5191 myeloid cells color-coded by their associated clusters. b t-SNE plot, color coding for the expression of the marker genes (gray to red) for the indicated cell subtype. c Heatmap of genes with differential expression (rows) among the myeloid cell subtypes. d Differences in pathway activities scored per cell by GSVA among the different myeloid cell subtypes. The scores of pathways are normalized. e Heatmap showing the activity of TFs in each myeloid cell subtypes. The TF activity is scored using AUCell. f Expression of BACH1 and RUNX1 in primary human monocytes and monocyte-derived macrophages. Data are presented as the means ± SEM of three independent experiments (n = 4). g Expression of NR1H3 and TFEC in primary human monocytes and monocyte-derived macrophages. Data represent the means ± SEM of two independent experiments (n = 4). h Primary human monocytes were transfected with negative control (NC), NR1H3-specific, or TFEC-specific siRNAs, followed by immunoblot analysis to determine protein expression of NR1H3 or TFEC. β-actin is the loading control. The experiments were repeated independently for three times with similar results. i, l Left, representative histograms of CD14 expression levels in siNC and siNR1H3 (n = 6 donors, n = 4 independent experiments) (i), and siTFEC (n = 6 donors, n = 4 independent experiments) (l) monocytes stimulated by M-CSF. Right, expression levels of CD14 in siNC and siNR1H3 (n = 6) (i), and siTFEC (n = 6) (l) monocytes stimulated by M-CSF, which was determined by FACS (MFI, mean fluorescent intensity). j, m Left, representative histograms of CD86 and HLA-DR expression levels in siNC and siNR1H3 (n = 6 donors, n = 4 independent experiments) (j), and siTFEC (n = 6 donors, n = 4 independent experiments) (m) monocytes stimulated by M-CSF. Right, expression levels of CD86 and HLA-DR in siNC and siNR1H3 (n = 6) (j), and siTFEC (n = 6) (m) monocytes stimulated by M-CSF, which was determined by FACS (MFI). k, n Kaplan–Meier curves showing progression-free survival in the 88 patients in NPC Cohort A stratified according to high vs low expression of NR1H3 (k) and TFEC (n). Cox regression HRs and 95% CIs obtained after correcting for age, sex, smoking history and disease stage are shown; the corresponding Cox regression P values are also shown. *P < 0.05, **P < 0.01 (paired Student’s t-test).