a, b Final viable cell densities (colony-forming units [CFUs/ml]) of R. palustris and E. coli and the final E. coli percentage (±SD) at generation 17 (a) and generation 146 (b) for WT-based (CGA4001, derivative of WT/CGA009 with inactive hydrogenase) and NifA*-based (CGA4003, derivative of NifA*/CGA676 with inactive hydrogenase) cocultures at the final time points shown in Fig. 2b, c. Points represent technical replicates of CFUs/ml for each lineage and lines are means, n = 3. The purple and orange lines in panel (b) indicate the median CFUs/ml for R. palustris and E. coli, respectively, at gen. 17 for reference. Data for lineage M at gen. 17 were unavailable due to agar contamination.