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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Sci Signal. 2020 Sep 15;13(649):eaau4518. doi: 10.1126/scisignal.aau4518

Fig. 1. Tumor exposure impairs the killing ability of CD8+ T cells.

Fig. 1.

(A) Experimental schematic for imaging the interaction of HA peptide–pulsed Renca tumor cells (Renca+HA) with retrovirally transduced, HA-specific, in vitro–activated CTLs in comparison to the same cells after adoptive transfer into mice with established Renca+HA cells tumors or co-culture with RencaHA spheroids. (B to D) Fluorescence microscopy analysis of the killing of CTV-labelled Renca or Renca+HA cells in vitro by Clone 4 CTLs or TILs. (B) Images are representative of 13 experiments. Scale bar, 100 μm. (C) The percentage changes in the area of Renca cells are means ± SEM from a minimum of three fields of view/time and are representative of seven experiments. (D) Average Renca+HA cell death rates are means ± 95% confidence interval from seven independent experiments. ****P < 0.0001 by Student’s t test with Welch’s correction.