Fig. 2. Fold changes of P. aeruginosa acoR, PA4148, liuA and zwf induced by culture conditions.
a, b Fold changes of acoR and PA4148 (A) and liuA and zwf (b) induced by co-culture with S. aureus (black bars), B. subtilis (grey bars), B. cenocepacia (hatched white bars) or S. maltophilia (hatched black bars). P. aeruginosa PA2600 strain was cultivated in the absence or presence of S. aureus SA2599, B. subtilis, B. cenocepacia or S. maltophilia. RNAs were extracted after 4 h of culture and gene expression was assayed by RT-qPCR. Bars represent the mean fold change + SEM from three independent experiments. Dotted lines indicate a fold change = |2|. *Padj < 0.05, **Padj < 0.01 and ***Padj < 0.001 ANOVA with Dunnett’s correction (S. aureus vs. condition). c, d Fold changes of acoR and PA4148 (c) and liuA and zwf (d) induced by culture in supernatant of S. aureus UAMS-1 wild type (WT, black bars), ΔalsSD mutant (white bars) and ΔalsSD mutant complemented with increasing acetoin concentrations (grey bars). P. aeruginosa PA2600 strain was cultivated in the absence or presence of filtered supernatant from S. aureus UAMS-1 WT, ΔalsSD or ΔalsSD complemented with acetoin concentrations ranging from 0.375 to 2 mM. RNAs were extracted after 4 h of culture and gene expression was assayed by RT-qPCR. Bars represent the mean fold change + SEM from three independent experiments. The dotted line indicates a fold change = |2|. *Padj < 0.05 and **Padj < 0.01 ANOVA with Tukey’s correction.