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. 2020 Aug 4;14(11):2794–2805. doi: 10.1038/s41396-020-0731-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Microscopy pictures of C. reinhardtii co-cultivated with or without A. nidulans and treated with or without azalomycin F. Size bars: 50 µm. b Autofluorescence of C. reinhardtii treated with azalomycin F (crosshatched bars) in monoculture (Control) and co-culture with A. nidulans. Loss of red autofluorescence indicates cell death. Scale bar 50 µm. b, c Autofluorescence of C. reinhardtii. b C. reinhardtii was either immediately treated with azalomycin F (−5 h) or allowed to associate with A. nidulans 5 h prior to azalomycin F addition (+5 h). c Correlation of protection of C. reinhardtii with an increased number of spores inoculated into the medium 18 h prior to addition of C. reinhardtii. Azalomycin F was added 5 h after coincubation. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ns not significant; data obtained from at least three biological replicates, error bars represent SDs.