Fig. 1.

Low expression of ISG12a in cancer cells and tissues. a, b Immunoblots showing levels of ISG12a in PHH, HLCZ01, LH86, Huh7, and HepG2 cells (a) or GES-1, BGC-823, and HGC-27 cells (b). Experiments were replicated three times, with similar results. c Immunoblots showing levels of ISG12a and Axin in representative CAs and para-CAs from patients with HCC or GC. d, e Scatterplots showing the relative ISG12a protein level between CA and para-CA from patients with HCC or GC. Protein expression in CAs and para-CAs examined by immunoblots is presented in Supplementary Figs. S1–S3. f Scatterplot showing the relative level of ISG12a mRNA between CA and para-CA from patients with HCC, as determined by qRT-PCR. g, h Representative images of IHC staining for ISG12a protein in CAs at different TNM stages (I, II, III, and IV) from patients with HCC or GC. Scale bar, 100 μm. Data were analyzed using two-sided Student’s t tests, and the results are presented as means ± SD (d–f)