. Author manuscript; available in PMC: 2021 Jan 5.

Published in final edited form as: ACS Catal. 2019 Oct 24;9(12):10721–10726. doi: 10.1021/acscatal.9b03990

Table 1.

Kinetic Constants for Cvβ3GalT and EcWbgO.

Enzyme	Substrate	k_cat (min⁻¹)	K_m (mM)	k_cat/K_m (min⁻¹ mM⁻¹)
Cvβ3GalT	LNT II-βProNHFmoc	(2.9±0.1) × 10²	0.74±0.07	3.9 × 10²
	UDP-Gal	79±4	0.23±0.06	3.4 × 10²

	GlcNAc	2.55	0.32	7.89
EcWbgO^a	LNT II	2.50	5.5 × 10⁻²	16.7
	UDP-Gal	13.90	3.4	4.09

Reported previously²⁸.