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. Author manuscript; available in PMC: 2021 Jan 5.
Published in final edited form as: J Biomed Mater Res B Appl Biomater. 2020 May 9;108(7):2981–2994. doi: 10.1002/jbm.b.34628

FIGURE 6.

FIGURE 6

Evaluation of cell viability and immunofluorescence staining of 3D bioprinted corneas. (a) Live dead assay in 3D printed corneas bearing human corneal keratocyte (HCK) cells on Day 1 (merged image). (b) Live dead assay in 3D printed corneas bearing HCK cells on Day 14 (merged image). (c) Alamar assay on 3D printed corneas on Days 1, 5, 10, and 14. Immunofluorescence staining of 3D printed corneas using a fluorescent microscope for (d) fibronectin (red), (e) F-actin (green), (f) DAPI (blue), and (g) merge image