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. 2021 Jan 5;9(1):e14606. doi: 10.14814/phy2.14606

FIGURE 4.

FIGURE 4

Unloading‐induced reduction of sarcolemmal neuronal nitric oxide synthase (nNOS) content in soleus muscle fibers is mitigated by losartan treatment. A (top row, second row), representative immunofluorescent images of nNOS stained soleus muscle sections at 10× and 40×. (third row), representative images of soleus muscle sections subjected to NADPH‐diaphorase (NADPH‐dia) histochemical assay to detect sarcolemmal nNOS activity taken at 10× magnification; (bottom row), NADPH‐dia images taken at 40× magnification. Scale bar = 100 µm. Scale bar = 100 µm. B, western blot detection of nNOS protein content in the membrane fraction and the cytosolic fraction. Ratio of cytosolic nNOS content to membrane content of nNOS based subcellular fractionation is presented in the accompanying graph. C, quantification of NADPH‐dia reactive staining found at the sarcolemma. Presented as percentage of cross‐sectional circumference (CSC) that is stained positive. Ponceau S Red (PR) stain (~42 kDa mark for membrane fraction; ~38 kDa mark for cytosolic fraction) used as a loading control for western blotting quantification. Values are presented as fold control of mean ± SEM. Values sharing the same letter are not significantly different (p < .05): aindicates different from CON; cindicates different from HUL; dindicates different from CONL