Figure 2.
(a) Effect of HJHR ameliorates mesangial cell pyroptosis in the contralateral kidneys of UUO rats through TUNEL. The rats were randomly divided into 4 groups: the sham group, the UUO group, the UUO with eplerenone group (100 mg·kg−1·day−1), and UUO with HJHR group. Renal sections were evaluated for TUNEL-positive cells. Sham: representative photomicrographs of contralateral glomeruli. UUO: representative photomicrographs of glomeruli from UUO rats. EPL: representative photomicrographs of glomeruli from UUO with eplerenone-treated rats. HJHR: representative photomicrographs of glomeruli from UUO with HJHR-treated rats. Positive cells showed brown staining. (b) Effect of eplerenone ameliorates mesangial cell injury by TUNEL. Human mesangial cells were treated for 4 groups: control group; Aldo group (1 μM Aldo); EPL group (10 μM EPL with 1 μM Aldo); HJHR group (HJHR with 1 μM Aldo). Equal numbers of cells were incubated for 24 h. (c) Quantitative analysis of TUNEL-positive cells in vivo and vitro.