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Inhibitory effect of HJHR on inflammation in the mesangial cells. (a) Western blot analysis of NLRP3, pro-caspase-1, pro-IL-1β, caspase-1-p20, and mature-IL-1β in the mesangial cells. (b) Quantification of NLRP3/GAPDH, pro-caspase-1/GAPDH, caspase-1-p20/GAPDH, pro-IL-1β/GAPDH, and mature-IL-1β/GAPDH protein band density in the contralateral kidney of sham, UUO, and EPL rats. Data represent the mean ± SEM (n = 3). ∗P < 0.05 vs. the sham group; #P < 0.05 vs. the UUO group.
