Figure 2.

CD4⁺ TILs colocalize with PD-L1⁺CD163⁺ TAMs. Representative multicolor immunofluorescence (multi-IF) images showing (a) high and (b) low colocalization of PD-L1⁺ cells with CD163⁺ TAMs. Scale bar, 100 μm. Representative multi-IF images showing costaining of (c) CD4⁺PD-L1⁺CD163⁺ TILs and (d) CD8⁺PD-L1⁺CD163⁺ TILs in serial sections of the same specimen. Cells were counterstained with DAPI (blue, nucleus). Scale bar, 100 μm.