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. 2020 Nov 4;28(1):1052–1062. doi: 10.1016/j.sjbs.2020.10.043

Table 2.

Quantitative phytochemical analysis of S. areira L. extracts.

Metabolite Reference compound Assay Reference RC Maceration
Soxhlet
HEX DCM EA MET AQ HEX DCM EA MET AQ
Carbohydrates Glucose Molisch Morsy, 2014 +++ +++ +++ +++ +++
Benedict Morsy, 2014 ++ ± +++ ++ +++ ++
Proteins and amino acids Albumin Ninhydrin Morsy, 2014 ++ ++ +++ ++ +++
Biuret Obasi et al. 2010 +++
Alkaloids Atropine Dragendorff Obasi et al. 2010 +++
Mayer Obasi et al. 2010 +++
Wagner Obasi et al. 2010 +++
Steroids and terpenoids Cholesterol Liebermann-Burchard Morsy, 2014 +++ + +++ ++ +++ ++ +
Salkowski Morsy, 2014 +++
Saponins Saponin Foam Morsy, 2014 +++ ++ + ++ +
Cardiac glycoside Digoxin Keller-Killiani Morsy, 2014 +++
Coumarin Warfarin Fluorescence Morsy, 2014 +++
Naphtho- and anthraquinones Bark of Rhamnus frangula, leaves of Cassia angustifolia M. Vahl Bornträger Morsy, 2014 +++
Bornträger (modified) Morsy, 2014 +
Flavonoids Quercetin Aluminium chloride Morsy, 2014 +++ ++ ++ ++ + ++ +
Shinoda Sarla et al., 2012 +++ + ++ + ++
Alkaline reagent Roopalatha and Vijay Mala, 2013 +++ + ++ +++ + ++ ++
Lead acetate Morsy, 2014 +++ +++ + +++ +++
Tannins and phenolic compounds Grape seed extract (Vitis vinifera) Iron(III) chloride Sarla et al., 2012 +++ ± +++ ++ ± +++ ++
Gelatine Sarla et al., 2012 +++ ± ++ ± ++

HEX, hexane extract; DCM, dichloromethane extract; EA, ethyl acetate extract; MET, methanolic extract; AQ, aqueous extract.

+++ = High, ++ = Moderate, + = Low ± = inconclusive; RC = result for the reference compound.