Table 2.
Quantitative phytochemical analysis of S. areira L. extracts.
Metabolite | Reference compound | Assay | Reference | RC | Maceration |
Soxhlet |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HEX | DCM | EA | MET | AQ | HEX | DCM | EA | MET | AQ | |||||
Carbohydrates | Glucose | Molisch | Morsy, 2014 | +++ | – | – | – | +++ | +++ | – | – | – | +++ | +++ |
Benedict | Morsy, 2014 | ++ | – | – | ± | +++ | ++ | – | – | – | +++ | ++ | ||
Proteins and amino acids | Albumin | Ninhydrin | Morsy, 2014 | ++ | – | – | – | ++ | +++ | – | – | – | ++ | +++ |
Biuret | Obasi et al. 2010 | +++ | – | – | – | – | – | – | – | – | – | – | ||
Alkaloids | Atropine | Dragendorff | Obasi et al. 2010 | +++ | – | – | – | – | – | – | – | – | – | – |
Mayer | Obasi et al. 2010 | +++ | – | – | – | – | – | – | – | – | – | – | ||
Wagner | Obasi et al. 2010 | +++ | – | – | – | – | – | – | – | – | – | – | ||
Steroids and terpenoids | Cholesterol | Liebermann-Burchard | Morsy, 2014 | +++ | + | +++ | ++ | – | – | +++ | ++ | + | – | – |
Salkowski | Morsy, 2014 | +++ | – | – | – | – | – | – | – | – | – | – | ||
Saponins | Saponin | Foam | Morsy, 2014 | +++ | – | – | – | ++ | + | – | – | – | ++ | + |
Cardiac glycoside | Digoxin | Keller-Killiani | Morsy, 2014 | +++ | – | – | – | – | – | – | – | – | – | – |
Coumarin | Warfarin | Fluorescence | Morsy, 2014 | +++ | – | – | – | – | – | – | – | – | – | – |
Naphtho- and anthraquinones | Bark of Rhamnus frangula, leaves of Cassia angustifolia M. Vahl | Bornträger | Morsy, 2014 | +++ | – | – | – | – | – | – | – | – | – | – |
Bornträger (modified) | Morsy, 2014 | + | – | – | – | – | – | – | – | – | – | – | ||
Flavonoids | Quercetin | Aluminium chloride | Morsy, 2014 | +++ | – | – | ++ | ++ | ++ | – | – | + | ++ | + |
Shinoda | Sarla et al., 2012 | +++ | – | – | – | + | ++ | – | – | – | + | ++ | ||
Alkaline reagent | Roopalatha and Vijay Mala, 2013 | +++ | – | – | + | ++ | +++ | – | – | + | ++ | ++ | ||
Lead acetate | Morsy, 2014 | +++ | – | – | – | +++ | + | – | – | – | +++ | +++ | ||
Tannins and phenolic compounds | Grape seed extract (Vitis vinifera) | Iron(III) chloride | Sarla et al., 2012 | +++ | – | – | ± | +++ | ++ | – | – | ± | +++ | ++ |
Gelatine | Sarla et al., 2012 | +++ | – | – | ± | ++ | – | – | – | ± | ++ | – |
HEX, hexane extract; DCM, dichloromethane extract; EA, ethyl acetate extract; MET, methanolic extract; AQ, aqueous extract.
+++ = High, ++ = Moderate, + = Low ± = inconclusive; RC = result for the reference compound.