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. 2020 Oct 29;141(1):67–83. doi: 10.1007/s00401-020-02239-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Demyelinating activity of rAB-hAE in vivo. Spinal cord sections of Lewis rats with MBP-specific T-cell induced experimental autoimmune encephalomyelitis, which have been intraperitoneally (a) or intrathecally (b) injected with rAB-hAE, r8-18C5 (positive control) or OCB-MS3-s1 (negative control). The tissues were stained with Luxol Fast Blue (LFB), antibodies against human IgG (brown, in case of weaker staining also arrowheads), against complement C9neo (red, in case of weaker staining also arrowheads), or with the ED1 antibody (brown). Bars 50 µm (first lane) or 100 µm (all other lanes)