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Primer sequences for step 1 in generation of antibody-repertoire specific libraries
| Primer pool name | Primer name | Sequence (5′–3′) |
|---|---|---|
| Reverse-transcription primer pool (10 µM) | HG_RT-in | AGAGGTGCTCTTGGAG |
| Cmu_RT-in | GGGAATTCTCACAGGAG | |
| Kappa_RT-in | CACACAACAGAGGCAG | |
| Lambda_RT-in | CACCAGTGTGGCCTTG | |
| Template switch oligo (10 µM) | TSO | /5Biosg/AAGCAGTGGTATCAACGCAGAGTACATrGrG+G |
