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. 2020 Oct 29;141(1):67–83. doi: 10.1007/s00401-020-02239-2

Table 2.

Primer sequences for step 2 in generation of antibody-repertoire specific libraries

Primer pool name Primer name Sequence (5′–3′)
TSO-PCR primer (100 µM) TSO-PCR primer AAGCAGTGGTATCAACGCAG
BCR specific primer pool (100 µM) unb_HG_IN new AGAGGTGCTCTTGGAG
unb_Cmu_OUT GGGAATTCTCACAGGAG
unb_Kappa_OUT CACACAACAGAGGCAGTTCC
unb_Lambda_OUT CACCAGTGTGGCCTTGTTGG