Table 3.
Primer sequences for step 3 in generation of antibody-repertoire specific libraries
| Primer pool name | Primer name | Sequence (5′–3′) |
|---|---|---|
| Nextera-TSO-primer pool (100 µM) | Nextera_NN_TSO-2ndPCR | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNCAGTGGTATCAACGCAGAG |
| Nextera_NNN_TSO-2ndPCR | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNCAGTGGTATCAACGCAGAG | |
| Nextera_NNNN_TSO-2ndPCR | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNCAGTGGTATCAACGCAGAG | |
| Nextera-BCR-primer pool (100 µM) | Nextera_NN_ IGHJ | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNGAGGAGACGGTGACCRKGGT |
| Nextera_NNN_ IGHJ | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNGAGGAGACGGTGACCRKGGT | |
| Nextera_NNNN_ IGHJ | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNNGAGGAGACGGTGACCRKGGT | |
| Nextera_NN_Kappa | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNGAAGATGAAGACAGATGGTGC | |
| Nextera_NNN_Kappa | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNGAAGATGAAGACAGATGGTGC | |
| Nextera_NNNN_Kappa | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNNGAAGATGAAGACAGATGGTGC | |
| Nextera_NN_Lambda | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNGCTTGGAGCTCCTCAGAGG | |
| Nextera_NNN_Lambda | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNGCTTGGAGCTCCTCAGAGG | |
| Nextera_NNNN_Lambda | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNNGCTTGGAGCTCCTCAGAGG |