Table 5.
Antibodies used for staining of rodent tissue
| Antibody | Source | Target | Antibody dilution | Pre-treatment | Secondary antibody (Jackson Immuno Research, Cambridge, UK) | Tyramine amplification |
|---|---|---|---|---|---|---|
| CNP | Sternberger Monoclonals Incorporated, Maryland, USA | 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (CNPase) present in oligodendrocytes and myelin | 1:2000 | EDTA 8.5 | Biotin-α-Mouse (1:500) | – |
| ED1 | VWR, Vienna, Austria | Rat CD68 protein expressed by macrophages and activated microglia | 1:10,000 | EDTA 8.5 | Biotin-α-Mouse (1:500) | – |
| C9neo | Details see [36] | activated complement component C9 | 1:2000 | Protease | Biotin-α-Rabbit (1:1000) | – |
| CD3 | Agilent, Cambridge, UK | CD3 on T cells | 1:1000 | EDTA 8.5 | Biotin-α-Rabbit (1:1000) | – |
| W3/13 | Abcam, Cambridge, UK | CD43 glycoprotein on T cells and neutrophils | 1:50 | Citrat | Biotin-α-Mouse (1:500) | – |
| Ig (Biotin-α-Human) | Szabo Scandic, Vienna, Austria | Human IgG and light chains of other human immunoglobulins | 1:1000 | Protease | – | – |