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. 2020 Dec 23;14:598678. doi: 10.3389/fncel.2020.598678

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Copyright © 2020 Masuoka, Yamashita, Nakano, Takechi, Niimura, Tawa, He, Ishizawa and Ishibashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photomicrographs of FM 1-43FX-positive corneal neurons and glial fibrillary acidic protein (GFAP)-immunoreactive cells in the anteromedial part of the trigeminal ganglion. (A,B) The trigeminal ganglion sections of the sham-operated (A) and gland excision guinea pigs (B). The corneal neurons were labeled with FM 1-43FX (green in the upper images), and the trigeminal ganglia were subsequently immunostained with anti-GFAP antibody (red in the middle images). The merged FM1-43FX/GFAP images are represented in the lower panels. The white arrows represent the FM 1-43FX-positive corneal neurons. Scale bars = 50 μm. (C) Percentage of FM 1-43FX-positive neurons completely (>80%) or partially (20–80%) enclosed by the GFAP-immunoreactive structure in the total FM 1-43FX-positive neurons. *P < 0.05 vs. the sham-operated group (Student’s t-test).