Figure 1.

Experimental design utilizing 42% high fat diet and Mogat1 targeted antisense oligonucleotides.

[A] Graphical representation of experimental design. After eight weeks on high fat diet, mice were given IP injections of a scrambled ASO (CTRL) versus Mogat1 ASO (ASO) twice a week for three weeks. [B] Representative H&E and Sirius red stained liver sections from sham operated CTRL and ASO treated mice following eight weeks of high fat diet and 3 weeks of CTRL or Mogat1 ASO injections along with high fat diet. [C] Hepatic gene expression of Mogat1 in CTRL and ASO treated mice following sham or IR surgery and indicated reperfusion times. [D] Representative protein immunoblot showing protein abundance of MGAT1 in livers of CTRL and ASO treated mice in sham or IR surgery and indicated reperfusion times. Values are mean ± standard error of the mean; n = 3–4 mice/sham group; n = 8–10 mice/IR surgery group. *p<0.05 ASO versus CTRL at same reperfusion time point. Abbreviations: ASO, antisense oligonucleotide targeted at Mogat1. AU, arbitrary units. CTRL, control ASO. HFD, high fat diet. IP, intraperitoneal. IR, ischemia reperfusion. H&E, hematoxylin and eosin.