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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Nov 7;534:864–870. doi: 10.1016/j.bbrc.2020.10.086

Figure 3. Suppression of Adipogenesis by ORM.

Figure 3.

(A) and (B) Differentiation of 3T3-L1 preadipocytes were induced by differentiation medium (MDI) with various concentrations of ORM protein (0, 125, 250, and 500 μg/ml). After 7 days, (A) Oil Red O staining of differentiated adipocytes. (B) Quantification of accumulated lipids by lysis of Oil Red O. (C) and (D) SVF preadipocytes from iWAT were differentiated (MDI) with various concentrations of ORM (0, 125, 250, and 500 μg/ml) for 7 days. (C) Visualization and (D) quantification after Oil Red O staining. Scale bar= 100 μm. *p<0.05, **p<0.01, and ***p<0.001 as compared to vehicle-treated differentiated cells.