Figure 3.

Basic function and drug metabolism in monolayer cultures (circles) or LAMPS chips (squares) that were seeded with (A) primary hepatocytes, or (B) iCell hepatocytes 2.0. Amounts of albumin, urea, LDH and TNFα were evaluated daily in the static cell culture media or perfusate collected from the devices. Control wells/chips (filled symbols) or wells/chips treated with daily additions with Terfenadine (10 μM, open symbols) were used in these experiments. (C) Drug metabolism capacity was evaluated by monitoring concentrations through addition of Terfenadine (10 μM, open symbols) to cell culture media or perfusate and formation of Fexofenadine (filled diamonds). Cell type and testing conditions are indicated in each chart. Data shown are mean±SEM (n=3–9 per condition).