Table 1.

Comparison of predicted human Terfenadine clearance in primary human hepatocytes and iPSC derived iCell hepatocytes determined from continuous perfusion over 10 days in the microfluidic 3D LAMPS, 48 hr bolus treatment in static 2D plate culture and 4 hr bolus treatment in suspension culture. All values represent mean ± SD.

Cell Type	3D LAMPS (ml/min)	2D Plate Culture (ml/min)	Suspension Culture (ml/min)
Primary human hepatocytes#	482 ±7 (n=4)	351 ± 6 (n=4)	1,172 ± 175 (n=2)
Human iCell Hepatocytes 2.0	516 ± 1* (n=3)	273 ± 10* (n=4)	771 ± 90 (n=2)

^#,For 3D LAMPS and 2D plate studies, human hepatocytes from the same batch of a single donor were used. For the 4 hr suspension cultures, human hepatocytes from a pool of 20 donors were used. All models used the same lot of iCell iPSC derived hepatocytes.

^*,Significantly different (p<0.05) from primary human hepatocytes in the same model by unpaired t-test (two-tailed).