Skip to main content
. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Nov 13;534:773–779. doi: 10.1016/j.bbrc.2020.11.001

Figure 2. HDAC8 inhibition or genetic loss induces caspase-dependent apoptosis of MCL cell lines.

Figure 2.

(A) HDAC8 inhibitor induced apoptosis. MCL cells were treated with 5μM PCI-34051 at seeding and 24 hours post seeding and Caspase3/7 activity was measured at 48 hours. (B) Genetic loss of HDAC8 induces apoptosis. Jeko-1 and Z138 cell lines were transduced with either non-targeting shRNA, or shRNAs specific for HDAC8 (#1 or #2). Apoptosis was measured 96 hours post transduction. The efficiency of HDAC8 knockdown is revealed by immunoblotting. Data is representative of 3 independent experiments and error bars represent the standard deviation. p-values (*p<0.05, *** p< 0.0001) represent significance compared to the NT control. The difference between shHD8#1 and shHD8#2 was not significant.