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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Nov 13;534:773–779. doi: 10.1016/j.bbrc.2020.11.001

Figure 4. Blocking HDAC8 activity preserves NK phenotype and cytolytic function.

Figure 4.

Analysis of NK receptor expression as median fluorescence intensity (MFI) (A) and the percentage of expressing cells (B) was assessed 24hours post PCI-34051 treatment. Human primary NK cells were isolated from PBMCs and treated with PCI-34051 for 1 hour prior to cytokine stimulation for 24 hours. Data represents four independent experiments with standard deviation indicated. (C) IFNγ secretion in response to HDAC8 inhibition. NK cells were treated as in panels A and B, and IFNγ was assessed by ELISA. Eight independent experiments are shown with the average and standard deviation indicated. (D) Percentage of IFNγ positive NK cells assessed by intracellular IFNγ flow cytometry. Cells were treated as indicated above. Each of the individual independent experiments are shown with the average and standard deviation indicated. *** p<0.0001 (E) NK cytolytic activity after HDAC8 inhibitor treatment. NK cells were co-cultured with K562 cells at an effector:target (E:T) ratio of 10:1. Each of the individual independent experiments are shown with the average and standard deviation indicated. (F) NK ADCC cytolytic activity. NK cells were co-cultured at an E:T ratio of 10:1 with Jeko-1 cells precoated with 10μg of Rituximab. Each of the individual independent experiments are shown with the average and standard deviation indicated.