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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Mol Cancer Res. 2020 Oct 8;19(1):136–149. doi: 10.1158/1541-7786.MCR-20-0554

Figure 5. Estrogen-like signaling is activated by a component bound to ECM.

Figure 5.

A, Schematic depicting experimental setup used for B-D. B-D, Immunoblots were performed using protein lysates from MCF7 cells plated onto ECM scaffolds (ECM) or uncoated surfaces with conditioned complete culture media from cancer cells (B), conditioned complete culture media from fibroblasts (C), or with disrupted decellularized ECM (D). Total Erk, phosphorylated Erk, AREG, TFF1, β-actin, and ER protein expression was detected.