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. 2020 Dec 23;11:607918. doi: 10.3389/fimmu.2020.607918

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Copyright © 2020 Steiner, Sotzny, Bauer, Na, Schmueck-Henneresse, Corman, Schwarz, Drosten, Wendering, Behrends, Volk, Scheibenbogen and Hanitsch

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating strategy for flow cytometry analysis of activated CD4+ and CD8+ T cells and their cytokine expression profile. Example of a gating strategy in a post coronavirus disease 2019 (COVID-19) healthy control (HC) in response to stimulation with the SARS-CoV-2 C-terminal spike peptide pool. Shown are (A) lymphocytes, (B) single cells, (C) living CD3+ T cells, (D) CD4+ and CD8+ T cells, (E) activated CD154+CD137+CD4+ T cells, (F–H) production of IFNγ (F), TNFα (G) and IL-2 (H) in CD154+CD137+ activated CD4+ T cells (I) and in CD137+CD8+ activated T cells (H–J) production of IFNγ (J), TNFα (K) and IL-2 (L) in activated CD8+ T cells. Single, double (dp) or triple (tp) cytokine producing activated T cell subsets were analyzed using Boolean combination gates.