Figure 1.

Thioflavin-S accumulation in spinal cord across life span. Spinal sections from the cervical cord of post-natal day 7 (P7) (C), P15 (E), P30 (G), and P45 (I) twitcher mice were stained with thioflavin-S (thio-S) to reveal presence of protein inclusions. There is a progressive accumulation across lifespan with the earliest detection at P7 primarily present only in the ventral horn (C, red arrows). A similar pattern of accumulation was noted within the lumbar horns at the same time-points P7 (D, red arrows), P15 (F), P30 (H), and P45 (J). Cervical (A) and lumbar (B) spinal sections from a P45 wild type (WT) mouse reveal the background staining and confirm a lack of thio-S reactive material. Images depicted are representative of the thio-S inclusion burden within the cervical and lumbar regions of the spine. They do not represent a precise spinal level. Scale bars represent 0.5 mm.