Figure 3.
Quantification and comparison of thioflavin-S accumulations across life-span. Stereological analysis of thioflavin-S accumulation density (inclusions/mm3) was performed. The ventral cervical and lumbar cords revealed large early accumulations at P7 and P15 with relatively slight additional accumulation over the final two time points (A,C). The dorsal spinal cord (B,D) showed a more protracted accumulation across life span. Psychopsine levels were measured in the cervical ventral (CV), cervical dorsal (CD), lumbar ventral (LV), and lumbar dorsal (LD) cord regions. This was compared across post-natal development (E) and in correlation to thio-s accumulation (F). (A–D) Significance between means analyzed by ANOVA and Tukey's post-hoc analysis with (*) indicating p < 0.05, (**) p < 0.01, and (***) p < 0.001, n = 3–4 animals. (E,F) Significance between means analyzed by ANOVA and Tukey's post-hoc analysis with (*) indicating p < 0.05 and (****) p < 0.0001 in comparison between CV and CD; (####) p < 0.0001 in comparison between LV and LD, n = 2–3 animals. Results are presented as mean ± error of the mean.