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. 2020 Dec 23;14:619712. doi: 10.3389/fncel.2020.619712

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Copyright © 2020 Marshall, Issa, Heller, Nguyen and Bongarzone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thioflavin-S accumulations present in neuronal cell populations of spinal cord. Spinal tissue from TWI (A) and WT (B) animals was stained with thioflavin (thio-S) and the neuronal marker NeuN, which found thio-S inclusions within neurons of TWI but not within neurons of WT animals. Dual-staining in TWI with thio-S (C) and alpha-synuclein (α-syn) (D) revealed colocalization (F) of a-syn with the thio-S inclusions. Cell nuclei are highlighted by DAPI (E). Scale bars represents (A–F): 20 μm.