Figure 5.

Alpha-synuclein staining in human Krabbe disease patients. Human spinal tissue from a patient with Krabbe (A–F) was stained with thioflavin-S (A,D) and for α-synuclein (α-syn) (B,E). Overlay of the two channels demonstrated colocalization of thioflavin-S reactive material with α-syn in cells of both the dorsal horn (C) and ventral horn (F). (G–I) [corresponding to the boxes in (D–F), respectively] show a cell with thioflavin-S and α-syn positive material as highlighted by the blue arrows compared to an unaffected cell highlighted by the white arrow. Cell comparison between panels is highlighted by white dashed line. Affected patients were both 2 years of age and in terminal stage of disease. Last, spinal tissue stained concurrently from an unaffected control case is shown in (J). Scale bar represents (A–F): 40 μm; (G–J): 20 μm.