FIGURE 2.

Detection of dying cells with TUNEL staining. (A) Central retinal TUNEL images from non-treated and treated with TRX wild type (WT) and rd1 mice at PN11, PN17 and PN28. (B) Quantification of TUNEL positive cells/arbitrary units of area (AUA) at PN11, PN17 and PN28 in three retinal regions (far periphery, medium and central retina). Mean values and standard errors are shown in the graph. The images are representative for observations for at least three different animals for each group (*p < 0.05 vs. WT vehicle treated, WT TRX treated and rd1 TRX treated; **p < 0.05 vs. WT vehicle treated and WT TRX treated). N = at least six in each experimental group. The TUNEL positive cells were counted manually in the outer nuclear layer (ONL) of three different parts of the retina: far periphery, medium and central retina. TUNEL positive cells from referred to the area of the ONL which was used.