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. 2020 Dec 23;11:615536. doi: 10.3389/fimmu.2020.615536

Figure 2.

Figure 2

SCFAs elevated oxygen consumption and HIF-1α gene expression in HKMs. (A, B) When HKMs were treated with NaB (10 mM), fluorescence of the oxygen probe in the cells was monitored within 2 h (A), and the rate of oxygen consumption at 2 h in HKMs was analyzed (B, n = 3). (C) Isolated macrophages were incubated with NaB (10 mM) for different time courses (0, 2, 8, 12, and 24 h), and HIF-1α gene expression in macrophages was detected (n = 3). (D) After HKMs were stimulated with 10 mM of NaB (n = 11), NaP (n = 6) or NaA (n = 6) for 24 h, the gene expression of HIF-1α in macrophages was measured. The results were representative of at least three independent experiments, and data were normalized by comparing to the control group. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.