Figure 3.

SCFAs promoted the gene expression and enzyme activity of lysozyme. (A, B) HKMs were stimulated with SCFAs (10 mM) for 24 h to analyze the gene expression of lysozyme (A, n = 7) and enzyme activity (B, n = 4). (C, D) HKMs were treated with NaB (10 mM) for 24 h. Thereafter, Edwarsiella tarda was added (cells: bacteria = 1:1) and co-incubated for 2 h, and the contents of ROS (C, n = 8) or NO (D, n = 8) in the cells were measured. The results were calculated from at least three independent experiments; error bars were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.