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. 2020 Dec 23;11:615536. doi: 10.3389/fimmu.2020.615536

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Copyright © 2020 Zhang, Zhang, Liu, Lan, Sun, Mai and Wan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sodium butyrate elevated antibacterial activity of RAW264.7 cells. RAW264.7 cells were cultured and stimulated with control buffer or NaB (1 mM) for 24 h. Afterwards, (A) the bacterial survival rate in RAW264.7 cells was analyzed (n = 8); (B, C) the protein level of HIF-1α in HKMs was assessed (B) and HIF-1α gene expression was also detected (C, n = 7); (D, E) The gene expression of lysozyme (D, n = 10) and lysozyme activity (E, n = 6) was measured. (F) The CAMP gene expression was analyzed (n = 8). The results were calculated from at least three independent experiments. Error bars represent mean ± SEM. *p < 0.05, ***p < 0.001.