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. 2020 Dec 23;8:596069. doi: 10.3389/fcell.2020.596069

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Copyright © 2020 Mignani, Zizioli, Borsani, Monti and Finazzi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphological analysis of embryos micro-injected with an ATG-blocking morpholino. Embryos were injected with the gene a-specific (ATG) or the control (STD) morpholino at 1/2 cell stage and analyzed at the microscope at 48 and 72 hpf. (A) Representative images showing the main features of controls and morphants at 48 hpf. Numbers in each panel represent total embryos used for the experiment and embryos with the phenotype shown in the image. (B) The graph shows the percentage distribution of embryos with normal or abnormal (mild and severe) morphology in each microinjection category. (C) The graph show the mean size of the eye diameter in controls and morphants (N > 40) at 48 and 72 hpf. Size bar = 500 μm. ****P < 0.0001 (unpaired, two-tailed T-Test). Experiments were repeated at least three times.