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. 2020 Dec 23;8:596069. doi: 10.3389/fcell.2020.596069

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Copyright © 2020 Mignani, Zizioli, Borsani, Monti and Finazzi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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neurod1-dependent EGFP fluorescence in embryos injected with control (STD) or ATG morpholinos. (A) Representative lateral and dorsal views of Tg(neurod1:EGFP) embryos either injected with STD or ATG morpholinos, at 48 hpf, Size bar = 500 μm. Experiments were repeated at least three times and altogether more than 150 embryos of each type were analyzed. (B) Lightsheet imaging of embryos described in panel (A). Four embryos with mild morphological phenotype were randomly selected and analyzed in two different experiments. Arrows point to the regions that are clearly missing or less visible in ATG-morphants.