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. 2020 Dec 23;8:584606. doi: 10.3389/fcell.2020.584606

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Copyright © 2020 Coccia, Masanas, López-Soriano, Segura, Comella and Pérez-García.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FAIM expression is modulated by miR-206, miR-1-3p, and miR-133b. (A) MiRNAs expression levels were assessed by RT-qPCR. (B) SK-N-AS, SH-SY5Y and HeLa cells were transfected with 25 nM of miR-140-3p, miR-206, miR-1-3p, miR-133b or a control miRNA (miR-Scr) and FAIM mRNA expression levels were assessed by RT-PCR. (C) Representative western blots of FAIM in neuroblastoma and HeLa cells transfected with the indicated miRNAs. Lower panels show the quantification of the band intensity of the western blots normalized to the control miRNA (miR-Scr) condition. *Unspecific band. Graphs represent the mean of four independent experiments ± SEM. Tubulin was used as a loading control. *P < 0.05, **P < 0.01, ***P < 0.005.