FIGURE 1.

Ishikawa diagram for quick visualization of the main categories of causes (plant material, cultural conditions, nutrition, plant growth regulators, and vitamins) that affect the plant cell tissue culture. Each category grouped factors representing the root causes of variation on the final quality of the process. As example of plant growth regulators. the next auxins (IAA, indole acetic acid; IBA, indole-butiric acid); CKs, cytokinins (BAP, Benzyl adenine purine; KIN, kinetin), and GA, gibberellins (GA₃, gibberellic acid) were included.