. 2020 Dec 23;11:554905. doi: 10.3389/fpls.2020.554905

TABLE 3.

Mineral nutrients’ (expressed as ions) composition of the different culture media based on the five-factor experimental design (0–33) and response values of the parameters (mean and standard deviation) used to characterize plant growth.

	Ions (mM)																		Growth parameters			Quality parameters

*Media*	NH₄⁺	NO₃^–	K⁺	Ca²⁺	Mg²⁺	PO₄^3–	SO₄^–2	Cl^–	Fe²⁺	BO₃^–	Mn²⁺	Zn²⁺	Cu²⁺	MoO₂^2–	Na⁺	Co²⁺	I^–	EDTA^–	SN	SL (cm)	LA (cm²)	SQ	BC	H
1	10.47	22.84	14.44	4.95	2.48	2.06	2.98	9.89	0.30	0.15	0.15	0.045	0.00015	0.0015	0.61	0.00016	0.0075	0.30	5.0 ± 0.8	1.6 ± 0.4	20.6 ± 6.3	3.8 ± 0.7	4.0 ± 0.0	2.9 ± 0.5
2	20.61	33.06	15.68	7.74	3.88	3.23	4.00	15.49	0.10	0.01	0.01	0.003	0.00001	0.0001	0.20	0.00001	0.0005	0.10	5.4 ± 2.7	1.7 ± 0.3	40.7 ± 10.9	3.2 ± 1.2	4.0 ± 0.0	1.8 ± 0.9
3	20.61	39.41	21.76	7.09	3.55	2.96	3.86	14.17	0.11	0.15	0.15	0.045	0.00015	0.0015	0.22	0.00016	0.0075	0.11	2.7 ± 0.8	2.3 ± 0.8	26.1 ± 12.8	4.4 ± 0.7	4.0 ± 0.0	2.8 ± 0.5
4	4.21	8.62	8.17	8.98	4.50	3.75	4.81	17.96	0.18	0.10	0.10	0.029	0.00010	0.0010	0.37	0.00010	0.0048	0.18	5.8 ± 2.6	1.9 ± 0.6	12.0 ± 4.0	3.5 ± 0.5	4.0 ± 0.0	1.8 ± 1.0
5	18.55	20.43	2.19	0.75	0.38	0.31	0.55	1.50	0.16	0.01	0.01	0.003	0.00001	0.0001	0.32	0.00001	0.0005	0.16	5.8 ± 1.6	1.2 ± 0.3	7.1 ± 3.4	3.1 ± 0.5	4.0 ± 0.0	3.0 ± 0.0
6	4.12	22.92	19.96	2.81	1.41	1.17	1.92	5.61	0.50	0.01	0.01	0.003	0.00001	0.0001	1.00	0.00001	0.0005	0.50	2.6 ± 1.1	1.2 ± 0.4	6.8 ± 3.2	2.5 ± 0.9	4.0 ± 0.0	3.0 ± 0.0
7	4.12	22.92	19.96	2.81	1.41	1.17	1.92	5.61	0.50	0.01	0.01	0.003	0.00001	0.0001	1.00	0.00001	0.0005	0.50	2.2 ± 1.2	1.4 ± 0.5	7.8 ± 4.4	2.2 ± 0.9	4.0 ± 0.0	3.0 ± 0.0
8	8.25	27.04	19.11	0.75	0.38	0.31	0.67	1.50	0.10	0.15	0.15	0.045	0.00015	0.0015	0.20	0.00016	0.0075	0.10	7.9 ± 2.7	1.1 ± 0.2	8.7 ± 3.9	2.8 ± 0.5	3.5 ± 0.5	2.9 ± 0.3
9	16.73	18.61	2.20	0.75	0.38	0.31	1.06	1.50	0.50	0.14	0.14	0.042	0.00014	0.0014	1.00	0.00015	0.0070	0.50	4.1 ± 1.5	0.7 ± 0.2	3.1 ± 1.7	1.7 ± 0.5	4.0 ± 0.0	3.0 ± 0.0
10	20.61	39.41	22.54	8.98	4.50	3.75	5.05	17.96	0.50	0.04	0.04	0.012	0.00004	0.0004	1.00	0.00004	0.0019	0.50	2.2 ± 1.1	1.3 ± 0.5	9.2 ± 3.6	2.3 ± 1.0	4.0 ± 0.0	2.2 ± 1.0
11	4.21	10.31	9.86	8.98	4.50	3.75	5.02	17.96	0.50	0.01	0.01	0.003	0.00001	0.0001	1.00	0.00001	0.0005	0.50	3.7 ± 1.1	1.0 ± 0.3	6.0 ± 2.1	1.7 ± 0.7	4.0 ± 0.0	1.9 ± 1.0
12	16.73	18.61	2.20	0.75	0.38	0.31	1.06	1.50	0.50	0.14	0.14	0.042	0.00014	0.0014	1.00	0.00015	0.0070	0.50	4.2 ± 1.5	0.8 ± 0.2	3.8 ± 1.2	1.5 ± 0.5	3.1 ± 0.2	3.0 ± 0.0
13	16.57	18.45	5.62	8.94	4.48	3.73	4.75	17.88	0.10	0.13	0.13	0.039	0.00013	0.0013	0.20	0.00014	0.0064	0.10	4.8 ± 1.0	1.7 ± 0.4	17.0 ± 5.5	3.6 ± 0.7	4.0 ± 0.0	1.9 ± 0.7
14	8.16	10.04	2.19	0.75	0.38	0.31	0.89	1.50	0.50	0.01	0.01	0.003	0.00001	0.0001	1.00	0.00001	0.0005	0.50	3.1 ± 1.4	0.7 ± 0.3	3.1 ± 1.3	1.0 ± 0.0	3.0 ± 0.0	3.0 ± 0.0
15	4.12	6.00	5.22	7.99	4.01	3.34	4.62	15.98	0.42	0.15	0.15	0.045	0.00015	0.0015	0.84	0.00016	0.0075	0.42	1.9 ± 0.8	1.1 ± 0.3	4.1 ± 1.8	1.3 ± 0.6	4.0 ± 0.0	2.9 ± 0.3
16	20.61	26.89	6.60	0.75	0.38	0.31	0.67	1.50	0.10	0.15	0.15	0.045	0.00015	0.0015	0.20	0.00016	0.0075	0.10	6.0 ± 1.2	1.5 ± 0.4	14.0 ± 3.4	3.1 ± 0.6	3.9 ± 0.2	2.6 ± 0.7
17	12.66	24.76	14.47	5.69	2.85	2.37	3.23	11.37	0.35	0.01	0.01	0.004	0.00001	0.0001	0.71	0.00001	0.0007	0.35	1.9 ± 1.1	1.2 ± 0.4	8.7 ± 2.3	2.3 ± 0.9	4.0 ± 0.0	3.0 ± 0.0
18	4.12	18.35	14.54	0.75	0.38	0.31	0.49	1.50	0.10	0.01	0.01	0.003	0.00001	0.0001	0.20	0.00001	0.0005	0.10	6.1 ± 1.4	1.3 ± 0.3	8.8 ± 2.8	2.9 ± 0.5	2.0 ± 0.0	2.9 ± 0.3
19	6.26	25.05	22.55	8.98	4.50	3.75	5.16	17.96	0.50	0.12	0.12	0.036	0.00012	0.0013	1.00	0.00013	0.0061	0.50	2.9 ± 1.5	1.7 ± 0.7	14.5 ± 6.4	3.4 ± 1.0	4.0 ± 0.0	2.7 ± 0.8
20	20.61	22.49	4.85	7.13	3.57	2.97	4.03	14.25	0.45	0.01	0.01	0.003	0.00001	0.0001	0.89	0.00001	0.0005	0.45	1.9 ± 0.9	1.5 ± 0.6	6.1 ± 3.7	2.1 ± 0.7	4.0 ± 0.0	2.9 ± 0.3
21	20.61	39.41	19.92	2.68	1.35	1.12	2.02	5.37	0.48	0.15	0.15	0.045	0.00015	0.0015	0.96	0.00016	0.0075	0.48	1.1 ± 0.3	1.7 ± 0.3	5.7 ± 3.4	2.3 ± 1.0	4.0 ± 0.0	3.0 ± 0.0
22	4.12	18.35	14.55	0.75	0.38	0.31	1.07	1.50	0.50	0.15	0.15	0.045	0.00015	0.0015	1.00	0.00016	0.0075	0.50	4.3 ± 0.9	1.0 ± 0.2	4.1 ± 1.4	1.7 ± 0.5	1.0 ± 0.0	3.0 ± 0.0
23	4.12	18.35	14.55	0.75	0.38	0.31	1.07	1.50	0.50	0.15	0.15	0.045	0.00015	0.0015	1.00	0.00016	0.0075	0.50	3.2 ± 1.3	1.0 ± 0.3	4.0 ± 1.6	1.7 ± 0.5	1.2 ± 0.7	3.0 ± 0.0
24	20.61	39.41	19.11	0.75	0.38	0.31	0.72	1.50	0.33	0.01	0.01	0.003	0.00001	0.0001	0.66	0.00001	0.0005	0.33	3.1 ± 1.5	1.3 ± 0.4	4.7 ± 2.0	1.9 ± 0.8	4.0 ± 0.0	2.6 ± 0.9
25	20.61	27.14	8.43	4.53	2.27	1.89	2.71	9.07	0.31	0.09	0.09	0.028	0.00009	0.0010	0.62	0.00010	0.0047	0.31	3.5 ± 0.9	2.2 ± 0.9	16.2 ± 5.3	3.7 ± 0.7	4.0 ± 0.0	2.9 ± 0.5
26	4.12	18.43	18.07	8.98	4.50	3.75	4.80	17.96	0.10	0.15	0.15	0.045	0.00015	0.0015	0.20	0.00016	0.0075	0.10	2.3 ± 1.0	1.9 ± 0.8	21.7 ± 10.3	4.2 ± 0.8	3.6 ± 0.5	2.8 ± 0.4
27	4.95	23.74	22.54	8.98	4.50	3.75	4.72	17.96	0.20	0.01	0.01	0.003	0.00001	0.0001	0.40	0.00001	0.0005	0.20	4.1 ± 1.8	2.0 ± 0.6	28.5 ± 8.5	3.9 ± 0.9	4.0 ± 0.0	1.4 ± 0.7
28	4.95	23.74	22.54	8.98	4.50	3.75	4.72	17.96	0.20	0.01	0.01	0.003	0.00001	0.0001	0.40	0.00001	0.0005	0.20	4.3 ± 1.5	1.9 ± 0.6	29.9 ± 13.8	4.1 ± 0.8	4.0 ± 0.0	1.8 ± 0.7
29	4.12	18.43	18.07	8.98	4.50	3.75	4.80	17.96	0.10	0.15	0.15	0.045	0.00015	0.0015	0.20	0.00016	0.0075	0.10	2.7 ± 1.0	1.8 ± 1.1	18.9 ± 9.3	3.5 ± 1.2	3.7 ± 0.5	2.4 ± 0.8
30	6.51	8.39	4.80	7.00	3.51	2.92	3.63	14.01	0.10	0.01	0.01	0.003	0.00001	0.0001	0.20	0.00001	0.0005	0.10	6.6 ± 2.4	1.1 ± 0.3	9.3 ± 3.9	1.4 ± 0.5	4.0 ± 0.0	1.0 ± 0.0
31	14.92	28.46	14.67	2.68	1.35	1.12	1.54	5.36	0.10	0.07	0.07	0.021	0.00007	0.0007	0.20	0.00007	0.0034	0.10	3.9 ± 1.4	2.6 ± 0.9	28.3 ± 9.3	4.7 ± 0.5	4.0 ± 0.0	2.7 ± 0.5
32	4.12	6.00	2.89	2.39	1.20	1.00	1.48	4.79	0.10	0.14	0.14	0.041	0.00014	0.0014	0.20	0.00014	0.0068	0.10	4.1 ± 1.7	1.2 ± 0.3	5.7 ± 2.9	2.4 ± 0.8	4.0 ± 0.0	2.9 ± 0.3
33	20.61	26.98	10.12	8.98	4.50	3.75	5.20	17.96	0.50	0.15	0.15	0.045	0.00015	0.0015	1.00	0.00016	0.0075	0.50	2.6 ± 2.1	1.5 ± 0.5	10.4 ± 5.5	2.6 ± 0.8	4.0 ± 0.0	2.4 ± 0.9
MS	20.61	39.41	20.05	2.99	1.50	1.25	1.73	5.99	0.10	0.10	0.10	0.030	0.00010	0.0010	0.20	0.00011	0.0050	0.10	3.9 ± 1.3	1.7 ± 0.4	28.7 ± 9.3	4.1 ± 0.4	4.0 ± 0.0	2.6 ± 0.6

Original medium composition (bold) used as control. SN, shoot number; SL, shoot length; LA, leaf area; SQ, shoot quality; BC, basal callus; and H, hyperhydricity.