TABLE 8.
Composition of the standard media (MS, Murashige and Skoog; B5, Gamborg; St, Standardi; Ha, -Harada) and the predicted optimized R medium together with output values obtained at validation experiment (mean ± standard deviation).
| R | MS | B5 | St | Ha | |
| Macronutrients (mg L–1) | |||||
| KNO3 | 1604.18 | 1900 | 2500 | 1800 | 1900 |
| NH4NO3 | 1297.09 | 1650 | – | 400 | 1650 |
| (NH4)2SO4 | – | – | 134 | – | – |
| Ca(NO3)2 4H2O | – | – | – | 1200 | – |
| CaCl2 2H2O | 545.61 | 440 | 150 | – | 440 |
| MgSO4 7H2O | 926.54 | 370 | 250 | 360 | 370 |
| KH2PO4 | 375.63 | 170 | – | 270 | 170 |
| NaH2PO4 H2O | – | – | 150 | – | – |
| Micronutrients (mg L–1) | |||||
| MnSO4 4H2O | 5.63 | 22.30 | 13.20 | 1.0 | 25.00 |
| ZnSO4 7H2O | 2.97 | 8.60 | 2.00 | 8.60 | 10.00 |
| H3BO3 | 5.07 | 6.20 | 3.00 | 6.20 | 10.00 |
| KI | 1.43 | 0.83 | 0.75 | 0.08 | – |
| CuSO4 5H2O | 0.05 | 0.025 | 0.025 | 0.025 | 0.025 |
| Na2MoO4 2H2O | 0.28 | 0.25 | 0.25 | 0.25 | 0.25 |
| CoCl2 6H2O | 0.04 | 0.025 | 0.025 | 0.025 | – |
| FeSO4 7H2O | 49.54 | 27.85 | 27.85 | 27.85 | 27.85 |
| Na2 EDTA 2H2O | 53.88 | 37.25 | 37.25 | 37.25 | 37.25 |
| Vitamins (mg L–1) | |||||
| Myo-inositol | 100 | 100 | 100 | 100 | 500 |
| Thiamine-HCl | 0.1 | 0.1 | 10.0 | 4.0 | 0.5 |
| Nicotinic-acid | 0.5 | 0.5 | 1.0 | – | 5.0 |
| Pyridoxine-HCl | 0.5 | 0.5 | 1.0 | – | 0.5 |
| Biotine | – | – | – | – | 0.05 |
| Folic acid | – | – | – | – | 0.5 |
| Glycine | 2.0 | 2.0 | – | – | 2.0 |
| Outputs values for the validation experiment | |||||
| SN | 3.6 ± 1.12 | 3.60 ± 1.01 | 3.51 ± 1.10 | 4.10 ± 1.16 | 4.04 ± 0.82 |
| SL (cm) | 2.69 ± 0.55b | 1.72 ± 0.40d | 1.88 ± 0.35d | 3.41 ± 1.20a | 2.10 ± 0.60cd |
| LA (cm2) | 34.06 ± 10.29bc | 29.58 ± 7.62cd | 21.43 ± 6.86e | 43.05 ± 8.57a | 38.39 ± 11.04ab |
| SQ | 4.51 ± 0.51a | 4.13 ± 0.7b | 3.22 ± 0.60c | 4.02 ± 0.27 ab | 3.87 ± 0.46b |
| BC | 4.00 ± 0.00a | 4.00 ± 0.00a | 3.00 ± 0.00c | 3.60 ± 0.50b | 4.00 ± 0.00a |
| H | 2.80 ± 0.46 | 2.64 ± 0.68 | 2.80 ± 0.55 | 2.67 ± 0.53 | 2.60 ± 0.69 |
Values with the same or without a letter are not significantly different at α = 0.001 (Tukey’s HSD Test). Predicted optimized R media composition and validated results are bolded. SN, shoot number; SL, shoot length; LA, leaf area; SQ, shoot quality; BC, basal callus; and H, hyperhydricity.