Fig. 3.

Effect of M-chlorin e6 PDT on the populations of M1-TAMs and M2-TAMs.

(A) Representative results of flow cytometric analyses on day 2 after LED irradiation. CD11b⁺, CD80⁻ or CD86⁻, CD206⁻ cells are defined as M0-TAMs. CD11b⁺, CD80⁺ or CD86⁺, CD206⁻ cells are defined as M1-TAMs. CD11b⁺, CD206⁺ cells are defined as M2-TAMs.

(B) Proportion of TAMs (CD11b⁺ cells) in live cells. Data are presented as mean ± SE (n = 8; Student's t-test).

(C) Proportion of M0-TAMs (CD11b⁺, CD80⁻ or CD86⁻, CD206⁻ cells) among all TAMs (CD11b⁺ cells). Data are presented as mean ± SE (n = 8; **p < 0.01; Student's t-test).

(D) Proportion of M1-TAMs (CD11b⁺, CD80⁺ or CD86⁺, CD206⁻ cells) among all TAMs (CD11b⁺ cells). Data are presented as mean ± SE (n = 8; *p < 0.05, **p < 0.01; Student's t-test).

(E) Proportion of M2-TAMs (CD11b⁺, CD206⁺ cells) among all TAMs (CD11b⁺ cells). Data are presented as mean ± SE (n = 8; **p < 0.01; Student's t-test).