Fig. 2.

Effect of LTCSIS in anxiety-like behaviour measured in the Novel object open field test. (A) time spent investigating the novel object (B) average speed (C) total distance travelled (D) percentage of time remained in the central zone (E) percentage of time remained in the corners. The data were analysed statistically using two-way ANOVA followed by Fisher's LSD post-hoc test. The statistical effect of stress treatment, sex and the interaction between the two factors are indicated in the top of the figure. Each symbol corresponds to data from a single-sex stress treatment group, represented as the mean ± SEM (n = 13). As is showed in Figures for two-way ANOVA crossing lines indicate the interaction between both factors: stress treatment and sex.