Fig. 3.

Effect of LTCSIS in anxiety-like behaviour measured in the Light-dark box test: (A) latency to the dark box measured as the time it took the animals to enter the dark part of the box. (B) the number of transitions measured as number of times the animals crossed from light to the dark box and vice versa. (C) total duration in light box measured as the amount of time spent by animals in the light compartment. The data were analysed statistically using two-way ANOVA followed by Fisher's LSD post-hoc test. The statistical effect of stress treatment, sex and the interaction between the two factors are indicated in the top of the figure. Each symbol corresponds to data from a single-sex stress treatment group, represented as the mean ± SEM (n = 13). As is showed in Figures for two-way ANOVA crossing lines indicate the interaction between both factors: stress treatment and sex.