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. 2020 Dec 23;10:584798. doi: 10.3389/fcimb.2020.584798

Figure 2.

Figure 2

Cell proliferation and cell cycle analyses of CRC cells pretreated with P. gingivalis. (A) CCK8 assay examination of the proliferation of S1 and MC38 cells pretreated with P. gingivalis 33277 and W83 for 24 h. (B) Colony formation assay examination of the proliferation of S1 and MC38 cells pretreated with P. gingivalis 33277 and W83. (C) Cell cycle analysis of S1 and MC38 cells pretreated with P. gingivalis 33277 and W83 detected by flow cytometry. The results showed that the fraction of S phase cells was significantly increased. *P < 0.05, **P < 0.01, ***P < 0.001. ns, nonsignificant.