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. 2020 Dec 23;14:600166. doi: 10.3389/fnins.2020.600166

FIGURE 2.

FIGURE 2

Male-specific blockade of contralateral AcGP by gonadectomy. (A) The surgical operation of orchiectomy or ovariectomy was performed 3 weeks before the first acid saline injection. Time points of acid saline injection into “Right” muscle and paw pressure test at the left paw were indicated by arrowheads and arrows, respectively. (B,C) Sexual dimorphic effects of ORX (B) and OVX (C) on the long-lasting mechanical allodynia after the second acid saline injection. Results represent the nociceptive paw withdrawal threshold (PWT in g) on the left side in the paw pressure test after the right acid saline injection (i.m.) in sham-operated or gonadal removed mice. Data at “post” were obtained at the day of 3 weeks after the gonadectomy. ∗∗p < 0.01, compared with each time point at Sham Cont, #p < 0.05; ##p < 0.01, compared with each time point at ORX Cont (B) or OVX Cont (C), †p < 0.05; †⁣†p < 0.01, compared with each time point at Sham AcGP, in two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test (Sham Cont, n = 4–5; Sham AcGP, n = 5; ORX Cont, n = 4, ORX Acid, n = 4; OVX Cont, n = 4; and OVX AcGP, n = 5). The dots in the column represent number of animals. AcGP, acid saline-induced FM-like generalized pain; ORX, orchiectomy; OVX, ovariectomy.