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. 2020 Dec 23;7:609055. doi: 10.3389/fvets.2020.609055

Table 1.

Proportion of polymorphonuclear cells (PMN) lymphocytes (Lym) and macrophages (Mac) observed in different studies, under different definitions of udder health statuses and using different methods of determination of these proportions.

References Definition Method PMN Lym Mac
(4) Healthy: no pathogen and SCC <10,000 cells/ml Cytology—Microscopy 10 67 23
1 day post-inoculation with Staphylococcus aureus Cytology—Microscopy 51 28 21
4–8 days post-inoculation with Staphylococcus aureus Cytology—Microscopy 39 38 24
9–14 days post-inoculation with Staphylococcus aureus Cytology—Microscopy 32 43 26
Healthy: no pathogen and SCC <10,000 cells/ml Flow cytometry 18 72 10
1 day post-inoculation with Staphylococcus aureus Flow cytometry 61 18 20
4–8 days post-inoculation with Staphylococcus aureus Flow cytometry 46 25 29
9–14 days post-inoculation with Staphylococcus aureus Flow cytometry 31 34 34
(5)a Healthy: SCC <100,000 cells/ml Flow cytometry 28 11 13
Acute E. coli Flow cytometry 38 1 4
Acute Staphylococcus aureus Flow cytometry 36 5 11
Chronic Staphylococcus aureus Flow cytometry 42 37 17
Chronic non-aureus Staphylococci Flow cytometry 49 18 13
Chronic Streptococcus dysgalactiae Flow cytometry 73 8 5
(6) SCC <50,000 cells/ml Flow cytometry 31 26 43
SCC between 50,000 and 100,000 cells/ml Flow cytometry 51 14 35
SCC between 100,000 and 200,000 cells/ml Flow cytometry 59 12 29
SCC between 200,000 and 400,000 cells/ml Flow cytometry 64 11 25
SCC >400,000 cells/ml Flow cytometry 67 9 24
(7) SCC <6,250 cells/ml Flow cytometry 15 63 22
SCC between 6,250 and 25,000 cells/ml Flow cytometry 17 59 24
SCC between 25,000 and 100,000 cells/ml Flow cytometry 23 50 27
SCC >100,000 cells/ml Flow cytometry 60 19 21
(8) SCC <6,250 cells/ml Cytology—Microscopy 12 56 32
SCC between 6,250 and 25,000 cells/ml Cytology—Microscopy 12 49 38
SCC between 25,000 and 100,000 cells/ml Cytology—Microscopy 52 31 17
SCC >100,000 cells/ml Cytology—Microscopy 78 12 10
(9) Healthy: No pathogen and SCC <100,000 cells/ml Cytology—Microscopy 43 34 23
Culture positive and SCC <100,000 cells/ml Cytology—Microscopy 56 23 21
Culture negative and SCC >100,000 cells/ml Cytology—Microscopy 63 16 21
Culture positive and SCC >100,000 cells/ml Cytology—Microscopy 68 12 20
(10)a Healthy: no pathogen and SCC <200,000 cells/ml Flow cytometry 28 58 9
Moderate mastitis but SCC <100,000 cells/ml Cytology—Microscopy 47 20 33
Moderate mastitis and SCC between 100,000 and 400,000 cells/ml Cytology—Microscopy 59 10 31
Severe mastitis but SCC <100,000 cells/ml Cytology—Microscopy 59 18 23
Severe mastitis and SCC between 100,000 and 400,000 cells/ml Cytology—Microscopy 71 9 20
Severe mastitis and SCC >400,000 cells/ml Cytology—Microscopy 82 5 13
(11) Healthy: low SCC and no pathogen Flow cytometry 43 30 27
(12) Healthy: SCC <100,000 cells/ml Flow cytometry 32 16 52
SCC >800,000 cells/ml Flow cytometry 49 18 33
(13) Healthy: no pathogen and SCC <100,000 cells/ml Flow cytometry 20 8 72
Infused with endotoxin Flow cytometry 78 2 19
(14) Healthy: no pathogen and SCC <100,000 cells/ml Cytology—Microscopy 28 25 47
a

Leucocyte proportions do not necessarily add to 1 because of measuring other cells in the original studies. The numbers were obtained from (6).