Supplemental Figure S8.

Pyruvate kinase M2 (PKM2) allosteric agent (TEPP-46) treatment reduces carbon tetrachloride (CCl₄)–induced mouse liver fibrosis. A: Schematic of the experimental design, and the structural formulas of TEPP-46. B: Representative images of mouse liver, hematoxylin-eosin (H&E), Sirius red, α-smooth muscle actin (α-SMA), and cytochrome P4502E1 (CYP2E1) staining. C: Ishak score, Sirius red–positive area, liver weight, and liver/body weight ratio of the indicated groups. D: Serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), albumin (Alb), and glucose (Glu) levels in the indicated groups. E: On-treatment weight change of each group. Results are presented as means ± SD (C–E). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus CCl₄; ^†P < 0.05, ^††P < 0.01, and ^†††P < 0.001 versus normal. Scale bars = 200 μm (B).